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Deeper penetration can access much larger volume in scattering tissue for in vivo imaging. The first part of this talk is focused on using Airy beams as the light source for deeper penetration. The propagation Airy beams is immune to diffraction and scattering, i.e. they can maintain their intensity profiles through a long propagation length, and preserve their intensity profiles after distortion. Nanometer resolution is needed to visualize molecular trafficking and interactions. Super resolution fluorescence microscopy has achieved great success since last decade. The second part of this talk is to develop super resolution microscope without molecular fluorescence using a femtosecond laser pump-probe method. Thick tissue growth in culture does not only require an appropriate scaffold for the cells to proliferate in, but also a microvasculature to transport nutrients and oxygen deep into the tissue. The last part of this talk will demonstrate using femtosecond lasers to fabricate 3D tissue scaffold which provide a structure for endothelial cells to grow into tubular monolayers, resembling the microcapillaries of our vascular system.
Dr. Chunqiang Li is an Associate Professor and Undergraduate Director in the Department of Physics at the University of Texas at El Paso. He graduated from Princeton University with his PhD in Electrical Engineering. His undergraduate and graduate studies were at the Chinese Academy of Sciences and Peking University in China. His current research interest in ultrafast laser spectroscopy and microscopy; cellular and molecular imaging in neuroscience and immunology.